The Roslin Institute

Genetics and Genomics

Porcine Genome Sequencing Project

Funded by: USDA CSREES, EC FP6 and others

The pig genome is being sequenced under the auspices of the Swine Genome Sequencing Consortium. A clone-by-clone hierarchical shotgun sequencing strategy was adopted exploiting the excellent clone-based (BAC contig) physical map of the pig genome. The Wellcome Trust Sanger Institute was the lead sequencing centre for this international collaborative project. The BAC clone sequence data were supplemented by whole genome shotgun (WGS) sequence data generated at BGI and Sanger from DNA from the same pig as the BAC clones. Funding is provided by USDA-CSREES, EC FP6 (SABRE), Institute for Pig Genetics and others. Draft sequences have been released as the project progressed with the first annotated version available in the Ensembl genome browser in 2009. A paper describing a high quality draft pig genome sequence will be published in 2012.

Relevant references:
Archibald, A.L., Bolund, L., Churcher, C., Fredholm, M., Groenen, M.A.M., Harlizius, B., Lee, K.-T., Milan, D., Rogers, J., Rothschild, M.F., Uenishi, H., Wang, J. and Schook, L.B. for the Swine Genome Sequencing Consortium, 2010. Pig genome sequence – analysis and publication strategy. BMC Genomics 11: 438.  www.biomedcentral.com/1471-2164/11/438

Humphray, S.J., Scott, C.E., Clark, R., Marron, B., Plumb, R., Bender, C., Camm, N., Davis, J., Jenks, A., Noon, A., Patel, M., Sehra, H., Yang, F., Rogatcheva, M.B., Milan, D., Chardon, P., Rohrer, G., Nonneman, D., de Jong, P., Meyers, S.N., Archibald, A., Beever, J.E,, Schook, L.B. and Rogers, J., 2007 A high utility integrated map of the pig genome. Genome Biology 8:R139. genomebiology.com/2007/8/7/R139

Schook, L.B., Beever, J.E., Rogers, J., Humphray, S., Archibald. A., Chardon, P., Milan, D., Rohrer, G. and Eversole, K., 2005. Swine Genome Sequencing Consortium (SGSC): a strategic roadmap for sequencing the pig genome. Comparative and Functional Genomics 6, 251-255.

Links:
Swine Genome Sequencing Consortium: piggenome.org/
Porcine Genome Sequencing Project (Sanger): www.sanger.ac.uk/Projects/S_scrofa/
Pig genome sequence in Ensembl: http://www.ensembl.org/Sus_scrofa/Info/Index
Pig physical (BAC contig) map in Pre-Ensembl: pre.ensembl.org/Sus_scrofa_map/Info/Index